Released N-Linked Glycan Analysis Employing Automated Sample Preparation

By Fang Wang, Sudha Savant, and Marcia Santos, SCIEX, USA; Beckman Coulter, USA

Review a streamlined N-linked glycan analysis that employs an automated sample preparation with the magnetic bead-mediated glycan workflow followed by capillary electrophoresis (CE).

A high-throughput N-linked glycan analysis can be efficiently achieved through the adoption of Biomek i5 multichannel automated workstation and the multi-capillary BioPhase 8800 system with the new Fast Glycan Labeling and Analysis kit. The inter- and intra-capillary reproducibility results demonstrate the robustness of the assay with automated sample preparation. Automated sample preparation of the release and labeling of N-linked glycans was developed and evaluated to demonstrate large-scale, reproducible screening on released N-linked glycans.