Poster

Promoter And PRE Triage For Gene Expression And Function Optimization

Source: ElevateBio

By Shen Lu, Christopher B. Driscoll, Kelsey Dupont, Emmanuel Nekongo, Alexander Boucher, Andrew Basinski, Elise Kim, Ana Maria Chadbourne, Cherylene A. Plewa, and Stacie L Seidel

Scientist in lab GettyImages-872025342

Optimizing lentiviral vector designs to increase titer, decrease production costs, and increase potency is an active area of exploration especially for vectors entering large scale manufacture. Here, we tested the effect of different promoters and PREs (Post-Regulatory Element) on multiple CAR and transgenic TCR constructs on titer, integration, expression, and in vitro function. To achieve this, we developed a scaled down mid-throughput lentiviral vector production platform to produce multiple constructs in tandem with sufficient titers for functional screening. Experiments were separated into two stages. In the 1st stage we triaged three unique MND promoters, and four types of PRE elements. The lead constructs were moved to the 2nd stage triage comparing EF1a, MSCV, MND, and SFFV promoters, for titer, transduction, expression and function using 4 genes of interest (GOI).

access the Poster!

Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Bioprocess Online? Subscribe today.

Subscribe to Bioprocess Online X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.

or

Subscribe to Bioprocess Online