Pipetting Procedures And Tips To Run An Accurate Assay

Even small variations in pipetting procedures may seriously impact assay results. This is of particular significance if the findings may be relevant for clinical or manufacturing decisions, as is the case with Limulus amebocyte lysate (LAL) assay used to assess samples for endotoxin contamination.

Learn about LAL assay, along with all steps of the pipetting procedure, including preparation, liquid uptake (aspiration), and liquid discharge should be considered to ensure the use of a proper technique. In addition to the described characteristics of a proper pipetting technique, adhering to the following tips would aid the delivery of consistent and reliable results.