Obtain Environmental Control Within The Detection Chamber For Cell-based Experiments

The investigation of biological processes requires time-dependent analysis of cellular signals, from several hours up to several days. However, long-term studies with living eukaryotic cells can only be performed for a limited time inside common microplate readers. This technical note describes the capability of Tecan’s Infinite® 200 PRO multimode reader to regulate the CO2 partial pressure inside its measurement chamber.

Tecan’s new patent pending Gas Control Module (GCM) is an innovative solution for cell-based experiments, offering rigorous environmental control within the detection chamber. Combining the patent pending Gas Control Module with a microplate that minimizes evaporation, such as the Thermo Scientific Nunc Edge plate, enables long-term cell-based kinetic measurements inside the reader, without negatively effecting cell proliferation and viability. Proliferation and survival of eukaryotic cells is strictly dependent on the environmental temperature and culture Although this is possible with some automated robotic systems, it is not ideal, and manual plate transfer will result in the measurement series being distorted by overnight gaps.

Although this is possible with some automated robotic systems, it is not ideal, and manual plate transfer will result in the measurement series being distorted by overnight gaps.