Chromatography Resin For Aggregate Clearance In mAb Purification: POROS™
Thermo Scientific™ POROS™ Caprylate Mixed-Mode Cation Exchange Chromatography Resin is designed to remove moderate to high levels of aggregates during downstream processing of therapeutic antibodies in flow-through mode. As a mixed-mode, weak cation-exchange resin, POROS Caprylate resin effectively removes other impurities, such as host-cell proteins and a wide range of high molecular weight species.
Features of POROS Caprylate Mixed-Mode resin include:
- Selective capture of aggregates and hydrophobic high-molecular-weight impurities
- Flow-through design that helps save buffer and material costs
- Excellent scalability and high productivity
- Wide operational range
- Non-animal-derived
POROS Caprylate Mixed-Mode resin operates in flow-through mode which reduces costs, especially when working with more engineered monoclonal antibody derivatives. The unique selectivity of the caprylate ligand contributes to a high yield of the targeted monomer while using established cation exchange resin buffer systems.
Main characteristics
- Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
- Average particle size: 50 μm
- Ligand: octanoic acid
- Binding capacity: >20 g lysozyme/L resin
- Typical buffers: typical cation exchange buffers, pH 4.5–7.0, conductivity 10–30 mS/cm
- Clean-in-place: 0.5 M NaOH
- Formulation buffer: 0.1 M NaOH
- Mechanical resistance: 100 bar (10 MPa, 1450 psi)
POROS Caprylate Mixed-Mode resin is manufactured using a synthetic manufacturing process that is free of animal components.