Cell Culture Vessels And Enhanced Attachment Microcarriers For Scale-Up And Production In The Vaccine Industry

By Katherine E. Strathearn, Ph.D., Mansi Soni, and Mark E. Rothenberg, Ph.D.

Viral transductions are used at the preclinical and clinical stages in the vaccine industry leading to an increase in demand to produce more virus more efficiently. The Corning® HYPER technology and microcarrier beads offer the ability to increase efficiency by increasing surface area without increasing spatial footprint. The focus of this study was (i) to determine the efficacy of generating Adeno- and lentiviral particles using the unique Corning HYPER technology, and (ii) to evaluate the use of the Enhanced Attachment microcarrier beads on cell lines typically used in the vaccine-producing industry (e.g. Vero, and HEK-293).