Hamilton Expands The pH Range Of Reversed-Phase Chromatography With Highly Efficient HPLC Column
Hamilton’s Laboratory and Sensor Products Division introduces the new Hamilton PRP™-C18 Reversed-Phase HPLC Column, designed to increase the accuracy and quality of separations performed by pharmaceutical companies, environmental scientists, and others.
WHITE PAPERS & CASE STUDIES
Alternative Technologies For Cell Culture Clarification Efficiency
Production of therapeutics and diagnostics by cell culture processes has become the cornerstone of the biotechnology industry. Cell culture systems can consist of bacterial, yeast, insect or mammalian cells, with mammalian cell culture production becoming the most widely used method. By 3M Purification Inc
Scaling Volume To Column Volume
In order to maintain the same elution pattern when scaling a separation between columns of the same phase but different inner diameters, gradient volume must be scaled in proportion to column volume.
Davisil® Technical Poster - 35Å Media for High Loading Adsorption This poster demonstrates that with 35Å silica-based high-purity irregular chromatography media, solvent savings of up to 45% can be achieved. Switching to the 35Å media also reduces related solvent disposal costs, which can lead to an overall cost savings in process-scale chromatography costs, taking in to consideration that solvent costs are actually much higher than media costs in the production process. By Oliver Genz, Thomas Pauly and Dr. Ian Chappell
Vydac® Reversed Phase Prep & Process Chromatography Application Note
Reversed-phase chromatography on Vydac® 300Å pore-size adsorbents is a very useful high-resolution method for protein separations, which is a powerful but often overlooked method for protein purification.
Vydac® Case Study – Scaling Gradient Volume To Column Volume
In order to maintain the same elution pattern when scaling a separation between columns of the same phase but different inner diameters, gradient volume must be scaled in proportion to column volume. In more complex reversed-phase separations of peptides of various sizes, changes in elution patterns may be more subtle but nonetheless problematic.
Vydac Tech Poster - Reversed Phase Scale-up
RP-HPLC is the preferred method for analyzing and purifying peptides and proteins. Pharmaceutical and biotech industries are working to optimize analytical separations of interest including polypeptides. By Reno Nguyen, Jochen Saar, Kim Vind, and Bart Denoulet