A Multiprotein Expression Strategy & Analysis
Detailed molecular analysis of multiprotein complexes is an emerging focus in the biological sciences. There is a growing demand for novel techniques enabling expression of multiprotein complexes. By Maxime Chaillet, Frederic Garzoni, Sinyee Yau-Rose, Barney Zoro, and Imre Berger
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Adaption Of The BioLector Technology To Anaerobic Requirements
Biorefineries have reignited interest in anaerobic fermentations with biobutanol production being the principle driver. Already during the First World War Biobutanol and acetone were produced in Clostridium acetobutylicum.
Batch Culture Of CHO Cells In The Multifors Cell
The Multifors Cell can be used to easily optimise development processes by parallel cultivation of animal cell cultures. Cultivation of the CHO (Chinese hamster ovary) cell line in the Multifors Cell bioreactor (INFORS HT, CH-Bottmingen) is described in the following as an example of batch cultivation of parallel samples.
Optimization Of Monoclonal Antibody Production Employing Noninvasive Sensor Technology
Large scale high density fermentation and high yield production of monoclonal antibodies are major aims of process development in biopharmaceutical industry.
Parallel Integrity Testing Of Small Venting Filters
Sterilizing grade venting filters are used to avoid the potential risk of air-borne germs contaminating containers like fermentors or cell-culture vessels.
Fed-Batch Biofuel Production Process In A Stirred-Tank Fermentor
The BioFlo 115 features a versatile and easy-to-use control station with color touchscreen monitor and builtin capability to operate in either fermentation or cellculture mode.
High Density Hybridoma Cell Culture In A Packed-Bed Bioreactor
Packed-bed bioreactor cell culture is generally accepted as one of the best methods to simulate the conditions of animal cell growth in vivo since cells are maintained in a low-shear environment with constant refreshment of nutrients and removal of waste. By Ray Rose, Stacey Willard, and Ma Sha
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Cell culture is a complex, highly structured process for growing cells, under strictly controlled conditions, outside of their normal environment. Cell cultures stilluse cultures of cells on flat plastic dishes.
This is referred to as two-dimensional (2D) cell culture. Aside from using Petri dishes for growing cells, scientists have for a long time, grown cells within biologically-derived matrices such as collagen or fibrin.
Today, more and more 3d cell cultures are being used because they more closely resemble the in vitro cell growth environment. Most 3d cell cultures in use today are designed for stem cell research, tissue engineering and drug discovery. As the field continues to grow and expand, 3d cell culture availability will likely expand to include other cell culture related fields.
For non-adhesive cells suspension cell cultures are used. In these cultures a cell is placed in the liquid suspension, stirred with a magnetic stirrer to agitate the cell and make it float freely in the suspension. The cell grows, divides and spreads throughout the suspension.
Cell culture refers to the culturing of cells derived from multi-cellular eukaryotes (cells with a nucleus), primarily animal cells. However cell cultures also exist for plants, fungi and microbes that include viruses, bacteria and microorganisms. Cell culture shares closely related methodology with tissue culture and organ culture.
You can separate cells from tissues for use in cultures several ways. Cells can be purified from blood but only white cells will grow in a culture. Mononuclear cells can be released from soft tissue using enzymes that break the cells away from their substrate or matrix. Pieces of tissue can also be placed in a growth media and the cells that grow from it can be used for cell cultures.