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FEATURED ARTICLES

  • A Multiprotein Expression Strategy & Analysis

    Detailed molecular analysis of multiprotein complexes is an emerging focus in the biological sciences. There is a growing demand for novel techniques enabling expression of multiprotein complexes. By Maxime Chaillet, Frederic Garzoni, Sinyee Yau-Rose, Barney Zoro, and Imre Berger

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Read-To-Use Medium: BalanCD™ MDCK

Read-To-Use Medium: BalanCD™ MDCK

BalanCD™ MDCK has been designed for producing viruses in Madin-Darby Canine Kidney cells (MDCK) cultures. This medium is chemically-defined and animal-component-free.

Flexsafe Single-Use Bags

Flexsafe Single-Use Bags

Standard Flexsafe 2D validation bags made of the new polyethylene S80 film are designed for small scale investigations and for the validation of the Flexsafe bag family. They are available in 150 mL and 500 mL volumes to support end users’ process development and validation testing. Flexsafe bags have been designed by combining material science and a Quality by Design approach to provide outstanding cell growth, robustness and assurance of supply for all process steps of biomanufacture.

BioLector® Pro

BioLector® Pro

The Biolector® Pro is the first microbioreactor system combining the proven, scalable BioLector® technology with an innovative microfluidic chip.

Animal Product Free Microcarriers

Animal Product Free Microcarriers

SoloHill Animal Product Free Microcarriers offer high performance options for researchers wishing to limit or remove animal sources from their processes. They address the worldwide concern with the spread of bovine spongiform encephalopathy (BSE) from cattle to humans as vCJD (variant Creutzfeldt-Jakob disease) which has driven the world's regulatory agencies to strongly suggest the eventual removal of all animal proteins from pharmaceutical manufacturing processes.

Minitron Incubator Shaker

Minitron Incubator Shaker

With the launch of the Minitron incubator shaker, INFORS HT is following up on the worldwide success of the Multitron incubator shaker. The Minitron has the same outstanding characteristics as its big brother, the Multitron, but on a smaller scale.

2D Bar Code Reader For Storage Vials

2D Bar Code Reader For Storage Vials

WHEATON understands that when it comes to BioBanking, sample integrity means everything.

Sample Batching And Storage Boxes

Sample Batching And Storage Boxes

WHEATON BioBanking Solutions include color coded storage and freezer boxes to make your sample storage and tracking solutions customized and easily identifiable.

Cryogenic Vials

Cryogenic Vials

WHEATON CryoELITE Cryogenic Vials offer unrivaled cap seals that exceed DOT and IATA regulations.

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Cell Culture
Cell culture is a complex, highly structured process for growing cells, under strictly controlled conditions, outside of their normal environment. Cell cultures stilluse cultures of cells on flat plastic dishes.
This is referred to as two-dimensional (2D) cell culture. Aside from using Petri dishes for growing cells, scientists have for a long time, grown cells within biologically-derived matrices such as collagen or fibrin.
Today, more and more 3d cell cultures are being used because they more closely resemble the in vitro cell growth environment. Most 3d cell cultures in use today are designed for stem cell research, tissue engineering and drug discovery. As the field continues to grow and expand, 3d cell culture availability will likely expand to include other cell culture related fields.
For non-adhesive cells suspension cell cultures are used. In these cultures a cell is placed in the liquid suspension, stirred with a magnetic stirrer to agitate the cell and make it float freely in the suspension. The cell grows, divides and spreads throughout the suspension.
Cell culture refers to the culturing of cells derived from multi-cellular eukaryotes (cells with a nucleus), primarily animal cells. However cell cultures also exist for plants, fungi and microbes that include viruses, bacteria and microorganisms. Cell culture shares closely related methodology with tissue culture and organ culture.
You can separate cells from tissues for use in cultures several ways. Cells can be purified from blood but only white cells will grow in a culture.  Mononuclear cells can be released from soft tissue using enzymes that break the cells away from their substrate or matrix. Pieces of tissue can also be placed in a growth media and the cells that grow from it can be used for cell cultures.