How Do I Get My Compound Into Phase I?
The primary challenge for pharmaceutical and biotechnology companies in developing their drugs is to carefully assess the relationship between efficacy and toxicity prior to entering into human clinical trials. By Dr. Scott E. Boley and Greg Ruppert
WHITE PAPERS & CASE STUDIES
High-Throughput Screening And Process Development For Capture Of Recombinant Pro-Insulin From E. coli
This Application note describes a complete process development workflow from PreDictor™ plate screening to a 400 mL pilot-scale purification for capturing recombinant pro-insulin expressed in E. coli.
Significantly Improve FoxP3 Staining In Human Whole Blood
Regulatory T-cells (Treg) play an essential role in the control of immune responses to self and foreign antigens maintaining the balance between immunity and tolerance.
Discovery Of Biochemical Markers For Understanding Disease
'Omic technologies and systems biology research provide a comprehensive view of the genetic, protein and biochemical nature of disease.
Automated Tissue Culture Cell Fixation And Staining In Microplates
Fluorescence microscopy has traditionally been performed on microscope slides, but there is a growing trend towards the use of 96- and 384-well microplates as this allows greater number of samples to be easily processed and automated. By Paul Held Ph. D., Bridget Bishop, and Peter Banks, Ph. D., BioTek Instruments, Inc.
How To Analyze Nuclear Stained Cells Using A Multi-Mode Microplate Reader With DAPI-Stained Cells
The imaging and analysis of fluorescently stained cells has traditionally been accomplished using manual microscopic methods with low numbers of samples, while the analysis of higher sample numbers requires the use of very expensive dedicated microscopes.
Automating Methods To Improve Assay Performance Suitable For Highthroughput Screening (HTS)
Here we demonstrate the use of a fluorescent polarization assay using SET7/9, a SET domain-containing mono-methyltransferase, as a model system suitable for HTS applications in a 384-well microplate format using automated methods and a HTS microplate reader.
Micro-Volume Determination Of Labeling Efficiency In A Standard Microplate Reader
A variety of methods and reagents have been developed to efficiently label biomolecules. By Peter J. Brescia, Applications Scientist, BioTek Instruments, Inc.
Automated Bioluminescent ADCC Reporter Bioassay Using Bioengineered Jurkat Cells
Pharmaceutical companies are increasingly exploring new biologic and biosimilar products, and thus increasing monoclonal antibody (mAb) immunotherapeutic research. By Tracy Worzella, Promega Corporation and Brad Larson, Applications Department, BioTek Instruments, Inc.
Assessment Of New Developments In ADCC Assays
With many of the existing patents covering antibody therapies set to expire in the next few years, the development of biologic therapeutics similar to the original drug (biosimilars) has become increasingly important.
Why Magnetic Beads Have Several Advantages Over Direct Coupling
The use of magnetic beads as the solid phase in ELISA (Enzyme-linked immunosorbent assay) has several advantages over direct coupling to the wells. These advantages include an increased available surface area and an even distribution of beads throughout the sample providing rapid and sensitive detection of low analyte concentrations (1. Liabakk et al).
Obtain Environmental Control Within The Detection Chamber For Cell-based Experiments
The investigation of biological processes requires time-dependent analysis of cellular signals, from several hours up to several days. However, long-term studies with living eukaryotic cells can only be performed for a limited time inside common microplate readers.
One Multimode Microplate Reader For All Essential Microorganism Growth Conditions
Tecan's multimode microplate reader automates growth monitoring of microaerophilic organisms.