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WakoPURE System

WakoPURE System is a proprietary protein synthesis & purification technology developed by Wako Pure Chemical Industries, Ltd. It is an in vitro protein synthesizing system "reconstituted" from translation factors expressed in E. coli. This unique system consists of around 30 purified enzymes necessary for transcription, translation and energy recycling. All components for transcription and translation, except for the ribosomes, are tagged with hexahistidine at the N or C terminus...
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Click here to download a brochure on the WakoPURE System in PDF format.

WakoPURE System is a proprietary protein synthesis & purification technology developed by Wako Pure Chemical Industries, Ltd. It is an in vitro protein synthesizing system "reconstituted" from translation factors expressed in E. coli. This unique system consists of around 30 purified enzymes necessary for transcription, translation and energy recycling. All components for transcription and translation, except for the ribosomes, are tagged with hexahistidine at the N or C terminus. Since these factors are tagged and known components, the synthesized protein can be easily purified by simply removing both the ribosome by ultra filtration, and the tagged factors and enzymes by metal affinity resin. The pure protein of interest can be synthesized and purified in about 3 hours.

Click here to download a brochure on the WakoPURE System in PDF format.


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