Products and Services
Pinpoint Slide RNA Isolation System II
- Easily isolates total RNA in any targeted microscopic paraffin-embedded tissue area on a slide.
- The simple procedure combines Pinpoint tissue sampling technology with a simple RNA extraction method.
- Efficiently recovers RNA from paraffin archive tissue sections for RT-PCR analysis.
General Description
- The Pinpoint Slide RNA Isolation System II is an innovative product designed by Zymo Research to easily isolate RNA from targeted paraffin-embedded tissue areas on a slide. The system combines our powerful Pinpoint tissue sampling technology with a one-step RNA extraction method and makes targeted tissue RNA isolation easy and simple. The kit efficiently recovers RNA from paraffin archives or fresh tissue sections for RT-PCR analysis. Unlike the UV-fractionation method, the Pinpoint system does not destroy the surrounding target cells. There is no need for expensive specialize equipment or computer software.
Method Principle: As outlined in Figure 1, simply apply the Pinpoint Solution to a selected microscopic tissue area. The solution will air-dry into a thin film while the underlying cells will become embedded within. The area is lifted and transferred into a tube. After digestion with Proteinase K the RNA is purified using the uniquely designed Zymo-Spin Column. The recovered RNA is ready for RT-PCR analysis.
Protocol
The Pinpoint Slide RNA Isolation System II is designed for extraction of RNA from paraffin-embedded tissue. The paraffin needs to be removed before starting this procedure. Although the Pinpoint Pinpoint Slide RNA Isolation II can also be used for fresh tissue RNA isolation, we recommend that you use the Pinpoint Slide RNA Isolation I, which is specialized for fresh tissue RNA isolation, Cat. No. R1003.
Zymo Research, 625 West Katella Ave, Suite 32, Orange, CA 92867-4619. Tel: 714-288-9682; Fax: 714-288-9643.
