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Zymed® NF_kB (p65) antibody (clone: 2A12A7, Catalog # 33-9900) is the preferred choice of some leading researchers for investigating NF_kB and its role in gene expression. In fact, this antibody was noted as being an "… awesome antibody…" and "…five times more sensitive than other p65 antibodies on Western blots…" (Dr. J. Donoto, Dept. of Cancer Biology, Cleveland Clinic). This antibody is useful for ELISA, Western blotting, and Gel Mobility Shift Assays.

The NF_kB family of induced-activated transcription factors are dimers comprised of members of the NF-_kB/rel protein family. NF_kB is present in the cytoplasm of most cell types as a 50 kDa (p50) subunit and a 65 kDa (p65, rel A) subunit. In unstimulated cells, nuclear localization signals present on p65 are masked by members of the I_kB family of inhibitory proteins. Subsequent to cell stimulation, I_kB undergoes phosphorylation, ubiquitination and degradation by a proteosome-dependent pathway, allowing nuclear translocation of the active dimeric NF_kB transcription factor. In the nucleus, NF_kB binds to consensus sequences, where, along with other co-factors, it participates in activating or enhancing the expression of specific genes.

Zymed Laboratories, Inc., 458 Carlton Court, South San Francisco, CA 94080. Tel: 650-8714494; Fax: 650-871-4499.

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