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Sorbents for Mixed-Mode, Hydrophobic Interaction Chromatography

PPA and HEA Hypercel are members of a family of Pall BioSepra chromatography sorbents designed for direct capture of proteins or contaminant removal from crude feedstreams. These sorbents bind proteins by a mixed-mode mechanism, principally hydrophobic interaction, operating in non-conventional, low-salt conditions (physiological HIC). They are based on the mechanically stable base matrix of Hypercel, currently used in the production of clinical material for late-stage clinical trials (MEP HyperCel).

PPA and HEA HyperCel facilitate binding from feedstocks or intermediate stage product that cannot be directly applied to traditional ion exchange or HIC sorbents:

• Conductivity is too high for traditional ion exchange sorbents
• Would require significant addition of salt for traditional HIC.
• Unlike traditional HIC, the target protein is recovered in dilute buffer, reducing the need for intermediate diafiltration.

Chromatographic behavior is based on a combination of electrostatic and hydrophobic properties of the protein and ligand:

• Can provide unique selectivities not accessible with traditional IEX or HIC.
• The differing retentivities & selectivities of PPA HyperCel, HEA HyperCel and MEP HyperCel can be screened to facilitate process development.

Features and Benefits

• PPA and HEA HyperCel sorbents offer an addition to the purification toolbox
• Different from ion exchange and conventional HIC
• High yield biological integrity
• No-salt capture limits risk of aggregation and denaturation
• Alternative and simple process scale HIC
• Accomplish binding using low-cost, environmentally-friendly salt
• Recover product in dilute or low conductivity buffer
• Reduced process costs

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