Products and Services
FFF- Field Flow Fractionation Separation System- Eclipse FFF System
The Eclipse is a state-of-the-art separation system using the asymmetric flow Field Flow Fractionation (FFF) technique. The Eclipse integrates easily with Wyatt's multi-angle light scattering (MALS) instruments: DAWN, miniDAWN, as well as the Optilab rEX. The combined FFF system provides absolute size and molar mass distributions of the particles/molecules separated by the AFFF unit. The combined FFF system solves the most difficult sizing tasks in the macromolecular and colloidal arenas. Its resolution is similar to sedimentation equilibrium, but it surpasses even this benchmark-sizing tool when one considers its flexibility, speed, and ease of use.
The Eclipse FFF provides unmatched separation power for samples in the sub-micrometer range. Distinct advantages of the Eclipse FFF over conventional "particle separators" or Hydrodynamic Chromatography (HDC) include:
- Ultra high resolution comparable to ultracentrifugation
- Flexible separation of inhomogenous samples
- Large dynamic range from a few nanometers up to a several micrometers
- Connects to modern detection instrumentation
- Fractions can be collected and used for off-line analysis (with electron microscopy, MS, ELISA etc.)
- Fully automated mode possible
- Turn-key system for asymmetrical Flow-FFF
- Built-in integration with DAWN detectors and ASTRA software
- Chassis stackable with Wyatt DAWN and Optilab rEX detectors
- Eclipse software controls HPLC pumps and autosample
- Channel is robust, but light-weight
- Operating pressure up to 30 bar
- Easy to change membranes and clean the system
- No dead volume in the channel, which minimizes system peaks
- Stainless steel frit, robust and corrosion free
- Extremely low surface area
•Brochure: FFF- Field Flow Fractionation Separation System- Eclipse FFF System
•Article: MALS + AFFF For Colloidal Drug Carrier Systems
•Article: Characterization Of Liposomes And Virus Particles With The Eclipse FFF Coupled To The DAWN EOS
•Article: Characterization Of Virus Like Particles By Asymmetrical Flow Field Flow Fractionation (FFF)
•Application Note: Aramide—Thermal FFF
•Article: Particle-Sizing/Field-Flow-Fractionation (FFF)
•Article: Peptide Hormone Aggregation By AFFF
•Article: Virus Particle Characterization
•Application Note: Liposome Characterization
•Application Note: Particle-Sizing/Lipid Emulsions
•Article: Characterization Of Branched Polymers By A4F-MALS
•Application Note: Size Characterization Of Glutenin Protein Polymers Using Field Flow Fractionation (FFF) And MALS Techniques
•Application Note: Virus-Like Particle Characterization Using New AF4 Channel Technology
