Products and Services

RSS

For Liquid Chromatography of Biologicals

DEAE and SP Spherodex® LS ion exchangers are stable composites comprised of two polymers: an inorganic silica matrix which provides a high degree of rigidity to support high flow rates, and an organic dextran component that provides hydrophilicity and bio-compatible functional groups.

The resulting interpenetrated polymer network has several advantages such as good chemical stability and bed stability in both aqueous and organic solvents. The sorbent does not shrink or swell with pH or ionic strength, and withstands pressure generated even when exceeding 600 cm/h. The unique network also eliminates non-specific sorption of proteins.

These sorbents were developed specifically for large scale process chromatography of therapeutic proteins and industrial enzymes. Spherodex ion exchange sorbents are presently used in column sizes from 20 to 3,000 L. SP Spherodex LS is supplied in wet form, in an aqueous suspension containing 1 M NaCl and 20% EtOH. DEAE Spherodex LS is supplied in dry form.

The most important properties of these ion exchange sorbents are:

• Stable bed at very high flow rate
• Stable to pressure (> 50 bar)
• Stable in acidic solutions
• Highly porous and hydrophilic
• Low non-specific adsorption
• No swelling or shrinking, due to aqueous or organic solvents

• Request Information
• Contact Details
• Company Profile