Nuvia media are a family of ultra high capacity ion exchangers designed for optimal performance in bioprocessing workflows. The media are built on a robust base matrix with proprietary surface extender technology. We are now offering Nuvia Q media, our exciting new high capacity anion exchanger delivering excellent resolution capabilities of feedstream contaminants such as host cell proteins and double-stranded DNA. Higher productivity can be achieved by using Nuvia Q through faster flow rates without compromising binding capacity. Moreover, less media is required and smaller columns can be used to purify a given amount of protein.

Dynamic Binding Capacity of Nuvia Q and Nuvia S

Dynamic binding capacity vs. flow velocity of Nuvia Q media.
Each 1.1 cm column was packed to a 10.6 cm bed height with Nuvia Q, agarose Q, or polymeric Q media. Five mg/ml BSA in 20 mM Tris-HCl, pH 8.5, was loaded onto each column until 10% breakthrough was observed. DBC BSA, dynamic binding capacity bovine serum albumin; BT, breakthrough.

Binding of polyclonal human IgG by Nuvia S media.
Comparison of Nuvia S and other commercially available CEX media. Column size, 0.7 x 5.5 cm. The sample was loaded onto the column in 40 mM sodium acetate, pH 5.0 + 30 mM sodium chloride, washed, and then eluted with 40 mM sodium acetate, pH 5.0 + 1 M sodium chloride. DBC, Dynamic binding capacity. BT, breakthrough.

The innovative design of the Nuvia S cation exchanger gives superior binding capacity across a broad range of pH, conductivity, and flow rate conditions. The unique performance characteristics of Nuvia S make it a flexible solution for both the capturing and polishing steps of protein therapeutics. Nuvia S is also an exceptional tool for removing contaminants such as mAb fragments and aggregates.

Get a taste of high-capacity performance.

Visit www.bio-rad.com/ad/nuviaq for a free Nuvia sample.

Downstream Purication Processes
- mAb
- Vaccines
- Biotherapuetics

Pharma/Biotech Focused Kinetic Screening Applications
- Antibody Screening
- Small Molecule Screening

Biomolecular Interaction Characterization
- Relative Ranking (Yes/No Binding)
- Kinetic Characterization
- Thermodynamics

Assay Development and Optimization
- Assay Development
- Assay Optimization

Testimonials:
"ProteOn XPR-36 proved to be very suitable by reducing the time needed to obtain reliable preliminary results" Marco Gobbi, Lab Manager, PharmD

"One of the main advantages of the ProteOn system is the ability to obtain a full kinetic dataset in just one injection" Simon Cocklin, PhD, Research Professor of Biochemistry and Molecular Biology, Drexel University, College of Medicine.

Webinars

Attend these free webinars to learn how the ProteOn XPR36 protein interaction system can help you to optimize, characterize, and screen with confidence. Simultaneously measure 36 molecular interactions in a single experiment and save time from setup to analysis.

October 27, 2011 8:00 AM (PST)

Identification of a novel ubiquitin binding domain by SPR

Presenter:
Adriano Marchese, PhD
Associate Professor,
Department of Pharmacology,
Stritch School of Medicine,
Loyola University Chicago

Host:
Laura Moriarty, PhD
Product Manager,
ProteOn Product Line,
Bio-Rad Laboratories

Ubiquitin is an important regulatory protein found in all almost all eukaryote tissue. Ubiqutin modification of proteins targets them for proteasomal degradation. It also serves many non-proteasomal functions in signal transduction, endocytic trafficking and DNA repair, among others. The functional outcome of ubiquitin modification is dependent upon non-covalent interactions with ubiquitin binding domains (UBDs). As many as 22 UBDs have been described and it is possible that other UBDs exist but this remains to be determined. Here we report an important novel UBD that shows a preference for polyubiquitin chains versus mono-ubiquitin. This finding may have significant impact for diseases where signaling is perturbed, such as cancer. We employed surface plasmon resonance (SPR) to characterize these interactions. This presentation will be followed by a live Q and A.

November 1, 2011 8:00 AM (PST)
November 1, 2011 8:00 PM (PST)

Applying the NEW high capacity HTE sensor chip for small molecule screening applications

Presenters:
Laura Moriarty, PhD and Ruben Luo, PhD
Product Manager,
ProteOn Product Line,
Bio-Rad Laboratories

Host:
Laura Moriarty, PhD
Product Manager,
ProteOn Product Line,
Bio-Rad Laboratories

In this webinar you will learn how the new high capacity HTE sensor chip from Bio-Rad has been used for the investigation of small molecule drugs binding to His-tagged targets. We will present how easy it is to achieve the high level of target immobilization required for this important application. You will also learn about other novel applications developed on the ProteOn XPR36 protein interaction array system and followed by a brief demonstration of the new ProteOn Manager software features. This presentation will be followed by a live Q and A with the presenters.

November 8, 2011 8:00 AM (PST)

Achieving high quality surface plasmon resonance data on the ProteOn XPR36 protein interaction array system

Presenter:
Jonathan Popplewell
Field Application Specialist, Bio-Rad Life Science, Europe

Host:
Laura Moriarty, PhD
Product Manager,
ProteOn Product Line,
Bio-Rad Laboratories

Attend this free webinar to learn how ProteOn users are generating high-quality and publishable surface plasmon resonance (SPR) data. This seminar will include information on how to generate and present publishable SPR curves and identifying and avoiding mass transport conditions. You will also learn about the new high capacity HTE sensor chip used for binding His-tagged proteins and how it's novel formulation can be used to produce exceptional results for small molecule analysis. A case study on small molecule binding to kinase targets will also be presented. This presentation will be followed by a live Q and A with the presenter.

November 16, 2011 8:00 AM (PST)

Functional and kinetic evaluation of bispecific albumin-binding domains binding a cancer related target using the ProteOn XPR36 platform

Presenters:
Johan Nilvebrant and Mikael Åstrand
School of Biotechnology,
Royal Institute of Technology (KTH),
AlbaNova University Center,
106 91 Stockholm, Sweden

Host:
Laura Moriarty, PhD
Product Manager,
ProteOn Product Line,
Bio-Rad Laboratories

A prominent challenge to overcome in the field of combinatorial protein engineering is functional screening of identified candidate molecules. The ability to achieve a full kinetic characterization during screening yields valuable insights. In this webinar, we will present our experience and methodology in characterizing bispecific affinity molecules. The binding molecules are derived from a combinatorial protein library based on a single albumin-binding domain selected to specifically bind a cancer-related receptor protein. In addition, a demanding competition assay is presented where selected binding proteins compete with the extracellular domain of the receptor for binding to an immobilized natural ligand. The presentation will be followed by a live Q and A.

View Webinar Series:

• Introduction to the ProteOn XPR36 Surface Plasmon Resonance (SPR) Interaction System
• Pushing The ProteOn SPR System To Its Limits

Events

Webinars

Videos

• The Impact Ion Exchange Media Has on the Operational Efficiency of Your Downstream Process
• New Tools and Alternatives to Streamline Your mAb Purification Process
• Antibody Characterization Using the ProteOn XPR36 System, a Multiplexed SPR Biosensor"
• Learn how Bio-Rad is evolving SPR to meet your needs – Featuring the new ProteOn ManagerTM Software and the new His-tag capture sensor chip
• Enhancing Throughput of the ProteOn Biosensor in Antibody Screening Applications
• An Introduction to the ProteOn XPR36 Surface Plasmon Resonance (SPR) Interaction System
• Epitope Binning Using the ProteOn XPR36
• Kinetic Screening of an scFv Antibody Fragment Library Using the ProteOn XPR36 Interaction Array System
• ProteOn XPR36 and Lipoparticle Technology, a Powerful Combination for Screening Antibody Therapeutics Againsted Membrane Proteins
• A Calcium-Dependent Immunocapture Strategy for Enhanced-Throughput SPR
• Novel Liposome Immobilization Using LayerLab Technology
• Pushing ProteOn to its Limits – From Protein-Protein Docking to Nanoparticle Interactions
• The Study of Small Molecule-Protein Kinase Interactions using Multiplexed SPR – Speeding Drug Discovery

• Bio-Rad Process Chromatography Introduction
• Bio-Rad Process Chromatography Skid 00
• Bio-Rad Control Console for InPlace Process Columns
• Bio-Rad InPlace Process Chromatography Column
• Packing CHT Chromatography Media In The Bio-Rad Inplace Column
• Bio-Rad EasyPack Process Chromatography Column
• Bio-Rad Process Chromatography Skid
• Bio-Rad Media Transfer Device
• Bio-Rad MainFrame Lifting Accessory