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Technical Bulletin: T045 Protein Purification Techniques, Volume 4, Metal-Chelate Affinity Chromatography

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Description

Metal-Chelate Affinity Chromatography (MCAC), also known as Immobilized Metal Affinity Chromatography (IMAC), was first successfully demonstrated in 1975 by Porath and collaborators for human serum proteins. MCAC utilizes metal ions bound to solid matrices that bind to the exposed imidazole and thiol groups of proteins. MCAC commonly utilizes zinc (Zn2+), nickel (Ni2+) or copper (Cu2+) to form stable complexes with histidine, tryptophan and cysteine residues within proteins.