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Technical Bulletin: T042 Protein Purification Techniques, Volume 3, Nucleic Acid Precipitation, proteolysis Inhibition and Lipid Removal
Source:
SAFC
Nucleic acids, lipids and proteases can often be in high concentrations in cell culture supernatants, contaminating the target protein. In addition, the method of extraction may increase the presence of these contaminants. Measures need to be taken to limit the effect of these contaminants on the downstream purification process.
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