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Technical Bulletin: T040 Protein Purification Techniques, Volume 1, Ionic Precipitation
Source:
SAFC
Protein precipitation achieves separation by the conversion of
soluble proteins to an insoluble state, which subsequently
can be removed by various means. Precipitation can be used
to remove components in cell culture media that may interfere
with downstream purification methods. Ideally, precipitation
results in both concentration and purification. Thus,
precipitation is often used early in the sequence of downstream
purification, reducing the volume and increasing the purity
of the protein prior to any chromatography steps.
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