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Feature Article: Regeneration Studies Of Anion-Exchange Chromatography Resins
By Paul K. Ng and Valerie McLaughlin
Chromatography columns can become contaminated by a variety of protein and nonprotein species during a purification campaign. Consequences of column contamination include an increase in backpressure, loss of signal resolution, altered product yield, and medium discoloration. Common chromatographic contaminants include
- residual proteins
- nucleic acids
- lipids
- endotoxins
- viruses and bacteria
- metal ions.
Generally, methods for cleaning-inplace (CIP) and sanitization-in-place (SIP) of chromatographic resins are selected based on the interplay and relevance of three factors: ease of operation, historical experience, and performance requirements. In most cases, a column decontamination method chosen by a laboratory forms the basis not only for process validation, but also for subsequent scale-up.
Reprinted With Permission From BioProcess International
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