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Application Note: Large-Scale Suspension Cell Growth And Secreted Product Yields Using THe Corning® HYPERFlask® Cell Culture Vessel

Corning Incorporated

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Description

By Kerry Spillane and Todd Upton, Ph.D., Corning Life Sciences

The Corning High Yielding Performance Flask (HYPERFlask) cell culture vessel was developed to meet increased needs for larger amounts of cells and secreted products such as antibodies by the pharmaceutical industry. With the same overall dimensions as the standard T175 flask, the HYPERFlask vessel is comprised of ten individual gas permeable layers, which allow for approximately ten times the total growth surface of a T175. As previously shown (see A Novel Flask Design for High Density Cell Culture), this increased growth area translates to ten times the total cell yield for adherent cell lines. We show here that similar yields are also achieved with both serum-free and serum-dependent suspension cell lines. Growth comparisons of CHO-S and MH-677 cells in the HYPERFlask vessel relative to a standard T175 flask showed no difference in cell density or viability. Further, the production of monoclonal antibodies (mouse IgG2a) by hybridoma cells was equivalent, per cm2 of growth surface, in both flasks, resulting in a 10-fold greater yield of cells from the HYPERFlask vessel.

The CHO-S cell line is a commercially available serum-free adapted clone of CHO cells and was selected to demonstrate the performance of suspension cells in serum-free media in the HYPERFlask vessel.