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Bulletin: Chimeric IgG Monoclonal Antibody Purification
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Bio-Rad Laboratories, Inc.
This tech note describes the purification of a chimeric IgG mAb using a two-step purification platform consisting of UNOsphere SUPrA™ medium capture followed by a CHT ceramic hydroxyapatite or CFT ceramic fluoroapatite media polishing step. The results demonstrate that both CHT and CFT deliver highly purified chimeric IgG by removing leached protein A, host cell protein (HCP), DNA, and mAb aggregates.
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