Sartobind STIC® — Salt Tolerant Interaction Chromatography Sartobind STIC® membrane adsorbers, from Sartorius Stedim Biotech, bind negatively charged impurities such as DNA, host cell proteins, endotoxins and viruses at much higher salt concentrations than known from conventional Q matrices. Sartobind STIC® membranes eliminate the need for feedstream dilution before flowthrough polishing of recombinant proteins and Mabs. This single-use product reduces validation cost, consumption of buffers, footprint and speeds up time to bring products to market. Learn more.
By Eric Langer, President and Managing Partner, BioPlan Associates, Inc.
Quality management is a matter of increasing importance to the biopharmaceutical manufacturing industry. In recent years, facilities have been gravitating toward quality-related analytical programs, and our data tells us they are also getting tougher on suppliers.
By Jennifer Camarda, Sales Specialist, Premium Balances and Software, Sartorius Corporation
As of December 1, 2013, significant changes to the USP Chapter 41, a mandatory chapter, became effective and now apply to all materials that must be accurately weighed. USP Chapter 41 has also been renamed, from Weights and Balances to Balances, and it does not reference the minimum sample weight anymore, but rather it defines repeatability and accuracy, which specifies the operating range of a balance. The operating range is limited to the maximum capacity of the balance and begins at the point at which the balance’s repeatability is less than or equal to 0.10 percent. The weighing must also be performed using a balance that is calibrated over its operating range.
By Michael V. Milburn, Karsten Suhre, Christa Meisinger, et al., Metabolon
Metabolomics is the rapidly evolving field of the comprehensive measurement of ideally all endogenous metabolites in a biological fluid. However, no single analytic technique covers the entire spectrum of the human metabolome.
By Richard Mirro, Eppendorf Inc.
This applications report presents a simple protocol for achieving high-density culture of Pichia pastoris (P. pastoris) cells using a New Brunswick benchtop, autoclavable stirred-tank fermentor or bioreactor.