Thermo Scientific Nunc InVitro Roller Bottles Outperform With ST, VERO, And MDBK Cells In Third Party TestingSource: Thermo Scientific Nalgene and Nunc
A third party study was conducted on the performance of Thermo Scientific Nunc InVitro roller bottles (1.2 and 2.5X) and competitive 850 cm2 roller bottles with ST, VERO, and MDBK cells. Cells were seeded at relatively low density into each bottle and allowed to grow to confluence. At confluence, cells were harvested and passaged at the same density into new bottles. This was repeated 4 times or until the culture failed to produce enough cells to seed. Confluency at 24, 48, 72, 96, and 120 hours was estimated visually. Cell counts per bottle (yield), as well as count per unit of surface area and percent viability, were determined at the end of each passage. Nunc™ InVitro™ roller bottles performed as well or better than the competitive. M-850 cm2 bottle with all cell lines tested.
A large proportion of vaccine production is devoted to doses administered to animals, and the majority of these are produced through a large scale cell culture system. Roller bottles made from polystyrene (PS) or polyethylene terephthalate (PETG) are commonly used in these culture systems as a growth substrate. The perception exists, however, that the cells used for such cell culture have been conditioned to grow on PS, and as such would either require acclimation to the new surface or fail to grow properly.