News | July 6, 2011
Thermo Fisher Scientific Responds to qPCR Webinar Interest with Launch of Informative Q & A Brochure
•The Future of qPCR: Best Practices, Standardization, and the MIQE Guidelines
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•Q&A Brochure: The Future Of qPCR Webinar: Best Practices, Standardization And The MIQE Guidelines
Thermo Fisher Scientific announced the availability of an informative brochure to address qPCR best practices and standardization issues. The brochure has been developed following Thermo Fisher's sponsorship of a qPCR webinar, which received an unprecedented amount of interest and resulted in a wealth of questions and requests for further information from viewers.
During the webinar, which was hosted by Science/AAAS,panelists discussed the important role that qPCR plays in research labs and diagnostics settings, as well as the technical challenges often associated with it. The questions that arose during the webinar ranged from very specific experiment-level queries to those that were about the broader qPCR topic. Responses to the questions were compiled and edited by the webinar panelists: Dr. Stephen Bustin, Dr. Gregory L. Shipley and Ms. Manju Sethi. The most frequently asked questions with the broadest applicability are addressed in the Q & A brochure and categorized under several topics ranging from contaminants and normalization genes to the MIQE guidelines and sample quantification.
The MIQE guidelines (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) specify a select number of products that will assist qPCR users to comply with requirements. The brochure provides guidance on the use of these products, including the Thermo Scientific NanoDrop 2000c UV-Vis spectrophotometer and Thermo Scientific Solaris qPCR assays. The guidelines state that template quantification is "essential" and an assessment of purity using spectrometry is "desirable". The microvolume capability of a NanoDrop™ 2000c facilitates rapid and easy purity assessment and an unprecedented range of concentration measurement (2 – 15,000 ng/µl for dsDNA) without the need for cuvettes.. Known probe sequences are also classified as "desirable" in the guidelines;the Solaris qPCR assay enables users to meet this requirement with its automatic inclusion of probe and primer sequence information with every assay.
"The webinar and subsequent Q&A session received overwhelming interest, demonstrating a real need for the availability of information on best practices and standardization in qPCR", said Dr. Andrew Page, Applications Manager of Thermo Fisher Scientific NanoDrop products. "Thermo Fisher is dedicated to assisting its customers and we hope that by addressing their most common questions and challenges, we will assist them in achieving high quality qPCR results."
To view the free, on-demand webinar, "The Future of qPCR: Best Practices, Standardization and the MIQE Guidelines" or for more information on the NanoDrop 2000c or Solaris qPCR assay, please visit www.thermoscientific.com/qpcrwebinar.
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of more than $10 billion, we have approximately 35,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through two premier brands, Thermo Scientific and Fisher Scientific, which offer a unique combination of continuous technology development and the most convenient purchasing options. Our products and services help accelerate the pace of scientific discovery, and solve analytical challenges ranging from complex research to routine testing to field applications. Visit www.thermofisher.com.
•The Future of qPCR: Best Practices, Standardization, and the MIQE Guidelines
Download Full Brochure:
•Q&A Brochure: The Future Of qPCR Webinar: Best Practices, Standardization And The MIQE Guidelines