Successfully Cultivate Hybridoma Cells In A Fibra-Cel® Packed-bed BasketSource: Eppendorf, Inc
The Fibra-Cel packed-bed basket technology has been established as an excellent method for the growth of suspension and anchorage-dependent cell lines. The three dimensional structure of the Fibra-Cel disk provides an excellent solid-support matrix for the entrapment or attachment of animal cells, allowing constant perfusion of nutrients in a low-shear environment.
Packed-bed bioreactor cell culture is generally accepted as one of the best methods to simulate the conditions of animal cell growth in vivo since cells are maintained in a low-shear environment with constant refreshment of nutrients and removal of waste. The growth of attachment-dependet cells on Fibra-Cel has been shown to increase both cell and product yields. In particular, Hybridoma cells are inherently sensitive to waste buildup and the implementation of packed-bed Fibra-Cel growth conditions in addition to perfusion production methods has greatly increased yields.
In this application note, we show that Hybridoma cells can be successfully cultivated in high densities in the 2.5 L packed-bed Fibra‑Cel basket controlled by a CelliGen 310 bioreactor.