Serum-Free & Chemically-Defined Media: IS CHO-CD™Source: Irvine Scientific
A versatile, chemcially-defined, animal component-free, serum-free media supporting robust growth and productivity in a variety of commonly used CHO clones. Useful with DHFR Selection System and GS Selection System.
IS CHO-CD™ is a chemically-defined medium optimized for the production of recombinant protein in Chinese Hamster Ovary (CHO) cells. The formula contains only defined components of non-animal origin. IS CHO-CD medium has been designed without Hypoxanthine, Thymidine or L-glutamine for use with the dihydrofolate reductase (dhfr), Glutamine Synthetase (GS) or other selection systems.
To address customer concerns relating to consistency, regulatory and downstream process issues, IS CHO-CD has been developed with components which are defined and of non-animal origin. This medium is also characterized for its ability to meet the different nutritional requirements of commonly used CHO clones. IS CHO-CD consistently outperforms competitor media in both growth and expression, allows complete adaptation from serum-free media, and demonstrates lotto-lot consistency.
• Components are chemically-defined and non-animal in origin.
• Contains no hydrolysates, extracts or other undefined components.
• Useful with dhfr selection system and GS selection system: does not contain L-Glutamine, Hypoxanthine or Thymidine.
• Supports high recombinant protein production.
• Shelf life of one (1) year when stored at 2-8°C and protected from light.
• Custom configurations are available.
• Available in 1L (liquid), 10L (powder) packaging
Direct Adaptation from Serum-Free CHO Media to IS CHO-CD
In many cases, CHO cells may be subcultured from a serum-free medium (e.g., IS CHO) directly into IS CHO-CD.
1. Dispense IS CHO-CD medium into an appropriate culture vessel and equilibrate to 37°C and 5% CO2.
2. Passage CHO cells from serum-free culture into IS CHO-CD at 3 X 105 viable cells/mL. It is important that cells be in the logarithmic phase of growth with at least 90% viability before passaging.
3. Incubate cultures at 37°C and 5% CO2 until the viable cell density reaches 1 X 106 cells/mL.
4. Subculture into fresh IS CHO-CD medium at 2 X105 cells/mL starting density.
5. Maintain cells in IS CHO-CD for several passages, subculturing twice weekly to allow complete adaptation and assure optimum performance.