Reducing Variability In Small Molecule Screening And Kinetics Applications

By Charles A. Wartchow, Ph.D. and Danfeng Yao, Ph.D.

Minimizing the variability of background signals is a key parameter to the success of demanding applications such as small molecule analysis. By reducing the background variability, smaller positive signals can successfully be resolved, providing an increase in assay sensitivity. This application note demonstrates the use of a reference biosensor with biotinylated streptavidin for increasing signal-to-noise levels, thereby improving the detection of small molecules, including fragments.