Minimizing Purification Bottlenecks To Flash Chromatography While Achieving Maximum Sample Recovery

By Scott Anderson, Kathy Lawrence, Dennis McCreary, and Melissa Wilcox

Purification bottlenecks encountered when using flash chromatography may include:

1. the necessity of collecting everything eluted during a run
2. checking the composition of every fraction collected, and
3. replacing an interfering solvent with a more compatible one.

By using the RevealX™ detection technology of the Reveleris® system, which independently triggers fraction collection from multiple detectors including UV and ELSD, this work will demonstrate that these bottlenecks can be reduced or eliminated without reducing the recovery of compounds of interest.  

To quantify the reduction in bottlenecks, a process map for flash purification was designed and tasks required assigned to each segment of the map. Time required for each of these tasks was then measured. Using these time measurements a comparison of total time required can be made between tradition flash technology using only UV detection and the RevealX™ technology of the Reveleris® system. The traditional flash technology is simulated by turning off the ELSD detector.