Linear Scalability Of Virus Production In Integrity® iCELLis® Single-Use, Fixed-Bed Bioreactors From Bench Scale To Industrial Scale
Over the past decade, single-use bioreactors have been developed and accepted for cGMP use in the bio¬manufacturing industry. Among them is the Integrity® iCELLis® series of bioreactors from ATMI LifeSciences, were designed for adherent cell culture.
Adherent cell processes for production of biomolecules and viruses often involve 2-D vessels (e.g. roller bottles, Cell Stacks/Cell Factories, etc.) requiring many manual operations and large areas of cleanroom space, or mi¬crocarrier cultures which require complex cell transfer steps and a very high level of expertise. Some cell culture processes allowing continuous production by medium perfusion also use adherent cells retained in a bioreactor while culture media is continuously perfused.
In order to maximize surface area within a compact space and retain cells for easy medium exchange, the iCELLis bioreactors contain macro-carriers trapped in a fixed-bed providing a 3-D matrix for the cells to grow. These bioreactors also enable precise temperature, pH and dissolved oxygen control which cannot be done in 2-D cultures.