Induction And Inhibition Studies Of Hypoxia And Oxidative Stress In Immortalized Keratinocytes

Hypoxic skin injuries are an important pathological process in many diseases. It has been shown that oxidative stress, the overproduction of reactive oxygen species (ROS), is intimately associated with hypoxic injury of skin. Here we demonstrate an in vitro multiplexed cellular imaging microplate assay that can monitor induction of hypoxia and oxidative stress. A luminescence cell viability assay was incorporated where cell viability measurements were performed in the same well following detection of the fluorescence signals from the Hypoxia/Oxidative Stress assay. All measurements were performed on the Cytation 3.