Improve Purification Of Carbohydrate Based DrugsSource: Grace
By Rakesh Bose, Scott Anderson, Kathy Lawrence, and Melissa Wilcox, Grace
Carbohydrates are commonly found as constituents in plants, animals, and microorganisms. Carbohydrate units have considerable applications in medicine when bound to lipids, peptides, proteins, or even nucleic acids. Total synthesis of bioactive natural products, amino glycoside antibiotics, glycodrugs, and glyco-conjugate vaccines from carbohydrates have been of considerable interest in the medicinal field for a cure. Due to their therapeutic effects, conjugation of carbohydrates and their presence in antibiotics can be effectively used to find cures for the treatment of various diseases. Besides their clinical importance in glycomics, synthesizing and purifying such molecules has been a challenge to synthetic chemists.
Separation and identification of carbohydrate-based compounds is complicated due to their structure and poor detection. Such drawbacks from lack of chromophores, high sensitivity, and the compound’s instability require a chromatographic system that is productive and efficient. As traditional fl ash chromatography is usually equipped with ultraviolet (UV) detection only, it fails to detect targets and impurities that are either present at low levels or lack chromophores. This study investigates the detection and purification of carbohydrates and glycodrug conjugates using RevealX™ detection technology, which incorporates multiple signal-processing from UV and ELSD (Evaporative Light Scattering Detection). Chemists can isolate and purify such compounds, which are non-chromophoric and used in medicinal drug discovery and development process, with speed and greater recovery.