HEA And PPA HyperCel™ Sorbents

Pall HEA and PPA HyperCel sorbents are industryscalable chromatography sorbents designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, their behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands.

HEA and PPA HyperCel sorbents provide unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography), that can be screened to facilitate process development.

For example, the mixed-mode interaction mechanism can be exploited to achieve discrimination of proteins isoforms, or proteins having similar or very close isoelectric points, separations which usually cannot be achieved by conventional methods. The sorbents’ mechanical stability allows their use at high flow rates in laboratory to production-scale columns.