Greater Loading Capacity And Resolution For Improved Productivity In Peptide Purification

By Dr. Reno Nguyen, Scott Anderson, Dr. Chitra Sundararajan, Dennis McCreary and Dr. Jochen Saar

Peptide therapeutics have been recognized as delivering the best of both worlds in terms of small molecule synthesis efficiency and biologics efficacy and reduced toxicity. However, challenges in mass production and low oral availability have historically made them less than ideal drug candidates. Recent advances in peptide synthesis technologies now enable manufacturing of complex peptides on a very large scale. In addition, emerging formulation and drug delivery technologies have improved the potency and oral delivery of peptide therapeutics.

Both of these developments have enabled new possibilities for peptide therapeutics. The process of synthesizing peptides however can produce many different side reactions and impurities. High capacity chromatographic media and purification strategies can increase loading can streamline manufacturing and increase production yields. This work demonstrates how the new Vydac® 150HC media, developed specifically for peptide purification, can improve peptide manufacturing productivity by increasing loading capacity.