Rapid Chemically-Defined Feed Media Development to Improve Simulated Cell Culture Processes

Media development is an integral step in optimizing a cell culture process. Modification to the growth medium in an existing process may not be a realistic option due to various constraints. Implementing an optimized feed medium may be the most effective alternative means of improving culture performance, whether starting from a batch or a fed-batch process.

To challenge this approach, feed media were developed to complement growth media with unknown formulations in simulated cell culture processes. The simulated processes consisted of two commercially available growth media paired with two CHO cell lines.