Engineering Characterization And Cell Culture Application Examples For Two Dimensional Orbitally Shaken Single-Use Bags
Engineering characterization is essential for successful scaling-up, in particular for geometrically dissimilar cultivations systems. The study provides an initial engineering characterization of orbitally shaken single-use bags with square footprints, using traditional methods, and is compared with geometrically dissimilar tubular and conical cultivation vessels such as TubeSpin and shake flasks. As an additional tool, Computational Fluid Dynamics (CFD) was used to gain a deeper insight and increase knowledge of fluid flow behaviour.
The results of the engineering characterization were validated by cultivating plant suspension cultures and insect cell cultures. Both, plant and insect suspension cells can be used for the production of bioactive substances such as secondary metabolites and recombinant proteins having importance in pharamacy and cosmetics. Thus, the option to easily scale-up processes using orbitally shaken bags shows great promise.