News | April 2, 2014

EMD Millipore Develops Simplified Protocol For Rapid Purification Of Recombinant Proteins

  • Combines lysis and purification into one step, resulting in significant time savings
  • Leads to more reproducible yields
  • Allows for automation using a high-throughput particle processor

EMD Millipore, the Life Science division of Merck KGaA of Darmstadt, Germany, recently introduced a new recombinant protein purification protocol that combines lysis and purification into a single step. The condensed workflow can be completed in about 45 minutes, reducing processing time by 75% compared to traditional techniques, while delivering high yield and purity.

The new protocol requires significantly less hands-on time than the conventional workflow, provides greater consistency and results in more reproducible yields. The method uses a specialized lysis buffer in combination with magnetic affinity capture beads, which together eliminate the need to clarify lysates by centrifugation. As a result, the workflow can be automated using a high-throughput particle processor, further reducing sample variability.

The one-step lysis and purification protocol has been validated specifically for EMD Millipore's PureProteome Nickel Magnetic Beads, optimized for the capture of histidine-tagged proteins, and EMD Millipore's BugBuster Master Mix, a lysis reagent that allows for non-mechanical extraction of soluble protein from bacterial cells. This reagent combines detergent-based lysis with enzymatic agents, Benzonase nuclease and rLysozyme enzyme, in a ready-to-use formulation.

"Protein chemists and those developing protein biotherapeutics crucially need a rapid, reproducible small-scale purification process," said David Briggs, Ph.D., Director, Product Marketing. "The traditional workflow takes more than three hours to complete, requiring separate lysis, lysate clarification and purification steps, which can negatively impact protein quality and reproducibility. Our new protocol offers a significant improvement, allowing researchers to purify recombinant proteins quickly and consistently."

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About EMD Millipore
EMD Millipore is the Life Science division of Merck KGaA of Germany and offers a broad range of innovative, performance products, services and business relationships that enable our customers' success in research, development and production of biotech and pharmaceutical drug therapies. Through dedicated collaboration on new scientific and engineering insights, and as one of the top three R&D investors in the Life Science Tools industry, EMD Millipore serves as a strategic partner to customers and helps advance the promise of life science.

SOURCE: EMD Millipore