White Paper | July 8, 2011

Dilution Of Samples, Issues To Consider

Source: Cygnus Technologies Inc.

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White Paper: Dilution Of Samples, Issues To Consider

By Cygnus Technologies Inc.

Some samples, particularly those from upstream in your purification process will have contaminant analyte concentrations above the analytical range of our very sensitive ELISA kits. Such samples may require very large dilutions in order to overcome "Hook Effect" and to achieve acceptable "sample dilutional linearity". Click on Poor Dilutional Linearity for a more detailed discussion on sample dilution issues. In addition to the "Hook Effect," the matrix of some samples may interfere non-specifically with the assay and also result in under recovery of the true analyte levels. Simple dilution of those samples is often adequate to buffer out such interference provided the dilution does not reduce analyte concentrations below the limit of quantitation of the assay. In cases where dilution of your samples is not an option contact our Technical Service Department for advice on how best to overcome sample matrix interference.

Cygnus offers assay specific diluents for each of its kits. The catalog numbers for these diluents can be found in the kit product insert or by contacting Customer Service. We strongly recommend use of those diluents because they are the same formulation as the matrix used for the kit standards. Thus, as you dilute your samples in our diluent, your sample matrix begins to approach that of the standards and in this way greatly minimizes any dilutional artifacts that could occur if you where to use another diluent.

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White Paper: Dilution Of Samples, Issues To Consider