Application Note | August 9, 2011

Tech Note: Culturing Primary Chick Embryo Cells On A Thermo Scientific Nunc Nunclon Cell Culture Treated Surface

Source: Thermo Scientific Nalgene and Nunc

Thermo Scientific Nunc Nunclon cell culture products are tested for cell growth and plating efficiency using several different cell lines.

Nunc™ Nunclon™ products are tested with two cell lines L929, HEL 299 or F2002 and one Primary Chick Embryo (PCE) cell culture for monolayer formation, plus cell line V79-4 for cloning efficiency.

Primary Chick Embryo cells are used to assess primary cell growth. This Tech Note describes a procedure for culturing cells isolated from chick embryos on a Nunclon treated surface.

Materials and Methods

  • Fertile eggs (10 to 12 day gestation)
  • Minimum Essential Medium Eagle (MEM)
  • Bovine Calf Serum (BCS), iron supplemented or Newborn Calf Serum (NCS)
  • L-Glutamine, 200 mm
  • Non-essential Amino Acids (NEAA), 100X
  • Dulbecco’s Phosphate Buffered Saline, 1X (without Ca2+ or Mg2+)
  • Trypsin Solution, 1X
  • Antibiotic/Antimycotic Solution, 100X
  • Crystal Violet or Methyl Violet, 0.1-0.4% in aqueous alcohol solution
  • Reference lot

 

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