Presentation: Comparison Of Results From Theoretical Sequence Search Engines And Peptide Mass Spectral Libraries For Selected Biological Samples

Summary

• Spectral library searching has many theoretical advantages over sequence searching
• Decoy libraries created from other species used with a simple target/decoy bias correction enable direct comparisons at FDR .01 with standard methods.
• Even at "low" coverage, library searching yielded equivalent or better results for real world samples.
• Library searching is an excellent addition to standard multiple search engine strategy.
• For known standards with high coverage, library searching is far more sensitive.