CHO Fed Batch Cultures: Overcome Barriers For High Concentration pH-Neutral Feed Supplements By Shawn L. Barrett, Ryan Boniface, Prasad Dhulipala, Mark Stramaglia, and Stephen F. Gorfien, Life Technologies
Robust fed-batch processes are required to leverage high cell-specific productivity and get the most out of bioreactor throughput. Current chemically defined fed-batch platforms for CHO-based production of recombinant proteins involve additions of one or more concentrated nutrient solutions.
Helium Integrity Testing: Prevent Worst-Case Scenario By Richard Bhella, Global PM, Single-Use Storage & Delivery Technologies, ATMI
Single-use manufacturing technologies have made significant inroads into the bioprocessing market in recent years, as companies recognize the advantages they offer in terms of saving time and money, and reducing the risks of contamination from one batch to another.
Spotlight On The Phacilitate Cell And Gene Therapy Forum
Filtration Processes Applied In Viral Vector-Based Gene Therapy Production Gene therapy involves the transfer of genetic material into a patient's cells in order to correct a disease state. The objective is to provide the necessary genetic material to correct a genetic deficiency and allow a particular therapeutic protein to be temporarily or permanently expressed. Candidates for gene therapy are often missing a particular gene, or have deficiencies in their genome, preventing them from producing fully functional, therapeutic proteins.
Capture Of Glycoproteins By Aminophenylboronate Affinity Chromatography Glycoproteins constitute a wide range of proteins, defined by having a carbohydrate motif as an inherent part of their structure. Some of these have been developed as therapeutic agents, such as erythropoetin, others exist as impurities in bioprocesses. In order to purify or remove these proteins at a commercial scale, chromatographic separation technology must invariably be employed.