Biotinylation Of Protein For Immobilization Onto Streptavidin Biosensors

The interaction between streptavidin and biotin is widely used as a system for the rapid, stable, and irreversible non-covalent binding of biological molecules. The Octet platform’s Streptavidin biosensors have been developed for the immobilization of biotinylated ligands for both quantitation and kinetic applications. The first protein immobilized onto the Streptavidin biosensors must be biotinylated prior to assaying on the Octet system.

This technical note supersedes ForteBio Technical Notes 6 (Biotinylation of Protein for Immobilization onto Streptavidin K Biosensors) and 12 (Biotinylating Very Small Quantities of Protein for Immobilization onto Streptavidin Biosensors). It provides guidelines for biotinylating a protein of interest utilizing Pierce- ThermoFisher (piercenet.com) biotinylation reagents for kinetic and quantitation assays with Streptavidin biosensors on the Octet platform.