Biochemical Characterization Of An Enzyme By Calculation Of Km And Vmax According To The Model Of Michaelis And MentenSource: Tecan
Enzymes play an important role in the organism by catalyzing (accelerating the rate of) and therefore regulating all metabolic reactions. Enzyme molecules bring the reactants into proximity, lower the activation energy, but are not consumed themselves in the reaction. The easiest enzyme mechanism is shown in equation 1. It involves the interaction of a single substrate molecule (S) with a single enzyme molecule (E) to reversibly form an enzyme-substrate complex (ES) and then an irreversible reaction to regenerate the uncomplexed enzyme and a product (P).