Application Note | April 28, 2014

Batch Culture Of CHO Cells In The Multifors Cell

Source: Infors Ag
ParallelCultures

The Multifors Cell can be used to easily optimise development processes by parallel cultivation of animal cell cultures. Cultivation of the CHO (Chinese hamster ovary) cell line in the Multifors Cell bioreactor (INFORS HT, CH-Bottmingen) is described in the following as an example of batch cultivation of parallel samples.

CHO cells are used quite commonly in biotechnology in the production of recombinant proteins. Cultures of the CHO XM-111 clone, transfected with an expression vector from the research group of Prof. Dr. M. Fussenegger at ETH Zürich, were carried out successfully in the Multifors Cell.

These cells contain the code for the gene of the recombinant SEAP protein (secreted alkaline phosphatase) which is controlled by the tetracycline-regulated PhCMV-1 promoter. The use of the expression vectors enables the expression of two genes by means of a single promoter that can be regulated. Accordingly, the SEAP production process consists of a non-productive growth phase, followed by a proliferation-inhibited production phase based on the depletion of tetracycline through a change of medium.

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