BalanCD® CHO Growth A Cell Culture Medium

BalanCD® CHO Growth A is a chemically-defined, animal component-free growth medium designed to increase process yields of antibodies and recombinant proteins in CHO cells. The formulation has been specifically designed to support growth and productivity in a variety of Chinese Hamster Ovary (CHO) cells in fed-batch cultures. BalanCD CHO Growth A was developed using Irvine Scientific’s Rational Culture Media Design™ strategy with three model cell lines (CHO DHFR, CHO-S, and CHO-M). This growth media was designed to be used in conjunction with Irvine Scientific’s BalanCD CHO Feed 1 to fully exploit the synergistic interaction between growth and feed medium in fed-batch systems.

Product Handling & Storage
Product handle using aseptic techniques is required to avoid contamination. Do not use if cloudy or if solution has precipitated. Powder product should be stored dry at 2-8°C and in absence of light for up to 2 years. Liquid product should be stored at 2-8°C and in absence of light for up to 1 year.

Related Products
The following products are available from Irvine Scientific and developed for use in CHO fed-batch production systems. For additional information, please visit our website at www.irvinesci.com or call Customer Service at (800) 427-5706.

Intended Use
These products are for research use only and not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro procedures.

Performance
Study Design
Three diverse high producing model cell lines (CHO DHFR, CHO-S, and CHO-M) were grown in multiple commercially available competitor growth media in batch mode and compared to BalanCD CHO Growth A. The following culture parameters were applicable to all experimental conditions

• Two (2) replicates per condition
• Shake flask cultures at 37°C, 5% CO₂, 120rpm