Automated Fluorescence Imaging Of GPCR Dependent Second-Messenger Systems

G-Protein Coupled Receptors (GPCRs) are the most notable initiators of the cell signaling cascade and are the targets of a large amount of the drug discovery effort. Here we describe the use of the Cytation 3 Imaging microplate reader to detect changes in second messenger levels using a series of genetically encoded biosensors to DAG, PIP2 , cAMP and Ca++ .