How To Analyze Nuclear Stained Cells Using A Multi-Mode Microplate Reader With DAPI-Stained Cells

By Paul Held Ph. D. and Peter Banks, Ph. D., BioTek Instruments, Inc.

The imaging and analysis of fluorescently stained cells has traditionally been accomplished using manual microscopic methods with low numbers of samples, while the analysis of higher sample numbers requires the use of very expensive dedicated microscopes. Here we demonstrate digital fluorescence microscopy using the Cytation™3, a novel imaging multi-mode microplate reader capable of both whole well intensity measurements using a PMT detection and digital inverted microscopy with a CCD detection. Gen5™ Data Analysis Software provides cell segmentation and counting in microplate wells. Onecolor staining of cells is performed in 96-well microplates and imaged using 4x and 20x microscope objectives.