Growing CHO Cells In A CelliGen® BLU Benchtop, Stirred-Tank Bioreactor Using Single-Use Vessels

The study presents a typical protocol for the setup and operation of New Brunswick Scientific's new CelliGen® BLU single-use, stirred-tank bioreactor, a versatile new benchtop system for the culture of a wide range of mammalian cells. This bioreactor has been designed to provide research and production facilities with a singleuse vessel which combines the benefits of both traditional stirred-tank design and single-use technology, capable of seamless process scale-up. The system can be operated in batch, fed-batch or continuous modes. A procedure for culturing Chinese Hamster Ovarian (CHO) cells in a 5.0 Liter (L) vessel, using CD CHO serum-free medium in a batch culture is described.