Davisil® Technical Poster — Natural Product PurificationSource: Grace
By Lori Sandford, Paul Garms, Theresa Riley and Jochen Saar
The reliable enrichment, analysis and purification of low abundance natural produce constituents from their complex crude extraction mixtures by liquid chromatography remains a key challenge in the pharmaceutical, food and flavors and natural products fields. Davisil® granular silica can bring substantial economy to the purification and enrichment of natural products and is shown to resolve difficult mixtures well at a large scale via normal and reversed phase HPLC. Natural products such as terpenes and terpenoids are a major factor in pharmaceuticals (paclitaxel), food and flavorings (citrus flavor) and microbiocidals (natural fungicides). Purification of terpenes and terpenoids is demonstrated on Davisil® C18 bonded silica for Paclitaxel extracted from Yew bark and on Davisil® normal phase silica for purification of the flavor and fragrance components, Limonene and a-Terpinene, from the essential oil of lemon. Vitamin E, Isomeric normal phase HPLC purification was developed that utilizes the substantial economy Davisil® offers through a two step purification strategy. The adverse affects of the ?-isomer on the chromatography of the desired ß-isomer under practical overload conditions were minimized by the two-step process resulting in a product that was greater than 93% pure.