Davisil® Technical Poster - 35Å Media for High Loading AdsorptionSource: Grace
By Oliver Genz, Thomas Pauly and Dr. Ian Chappell
In traditional chromatographic purification and manufacturing processes of pharmacological small molecules, (active pharmaceutical ingredients = API) separation media with a 60Å pore size are very commonly used. A pore size of 60Å in the separation media particle allows small molecules (MW 150–300) to enter the pores easily and generally leads to good chromatographic separations.
35Å silica chromatography media, however, has an improved surface area compared to comparable 60Å silica separation media. This can lead to a significantly higher loading capacity in process scale purification. In addition to the solvent savings, this can reduce chromatographic manufacturing costs by more than 50% in total.
This poster demonstrates that with 35Å silica-based high-purity irregular chromatography media, solvent savings of up to 45% can be achieved. Switching to the 35Å media also reduces related solvent disposal costs, which can lead to an overall cost savings in process-scale chromatography costs, taking in to consideration that solvent costs are actually much higher than media costs in the production process.